mouse monoclonal anti hsp47  (Novus Biologicals)

Journal: Scientific Reports

Article Title: Machine learning-based construction of Immunogenic cell death-related score for improving prognosis and personalized treatment in glioma

doi: 10.1038/s41598-025-15658-6

Figure Lengend Snippet: Expression and prognostic analysis of SERPINH1 in gliomas. ( A-E ) The expression levels of SERPINH1 in the GTEx & TCGA_LGG ( A ), GTEx & TCGA_GBM ( B ), GSE50161 ( C ), GSE29796 ( D ), and GSE4290 ( E ) datasets. ( F-I ) The expression levels of SERPINH1 in different grades of gliomas within GSE4290 ( F ), CGGA_301 ( G ), CGGA_325 ( H ), and CGGA_693 ( I ) datasets. ( J-N ) KM curves of the OS of glioma patients in the CGGA_301 ( J ), CGGA_325 ( K ), CGGA_693 ( L ), GSE74187 ( M ), and TCGA_LGG ( N ) datasets.

Article Snippet: Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence were performed using primary antibodies against SERPINH1 (proteintech, 10875-1-AP, China), MYD88 (proteintech, 23230-1-AP, China), LY96 (proteintech, 11784-1-AP, China), PDIA3 (proteintech, 15967-1-AP, China), GAPDH (proteintech, 80570-1-RR, China).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Machine learning-based construction of Immunogenic cell death-related score for improving prognosis and personalized treatment in glioma

doi: 10.1038/s41598-025-15658-6

Figure Lengend Snippet: SERPINH1promotes glioma cell proliferation and migration in vitro. ( A-D ) The qPCR and WB assays validate the efficacy of SERPINH1 silencing by siRNA in U251 and LN229 cells. Because the better silencing effect of siSERPINH1*1, it was used in all subsequent experiments. ( E-F ) CCK8 assays evaluate changes in cell proliferative capacity after SERPINH1 knockdown in U251 and LN229 cells. ( G-K ) Flow cytometry assesses alterations in the cell cycle subsequent to SERPINH1 knockdown in U251 and LN229 cells. ( I-L ) The transwell assays assess the alterations in the migration capabilities of U251 and LN229 cells following SERPINH1 knockdown. ( M-N ) The EdU assays assess changes in proliferative ability of U251 and LN229 cells after SERPINH1 knockdown. ***, p < 0.001.

Article Snippet: Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence were performed using primary antibodies against SERPINH1 (proteintech, 10875-1-AP, China), MYD88 (proteintech, 23230-1-AP, China), LY96 (proteintech, 11784-1-AP, China), PDIA3 (proteintech, 15967-1-AP, China), GAPDH (proteintech, 80570-1-RR, China).

Techniques: Migration, In Vitro, Knockdown, Flow Cytometry

Journal: Scientific Reports

Article Title: Machine learning-based construction of Immunogenic cell death-related score for improving prognosis and personalized treatment in glioma

doi: 10.1038/s41598-025-15658-6

Figure Lengend Snippet: SERPINH1 promotes JAK/STAT signaling pathway in glioma. ( A ) Patients in the CGGA_325 cohort were divided into two equal groups based on the median SERPINH1 level, then underwent GSEA. ( B ) Knockdown of SERPINH1 in glioma cells by siRNA was confirmed by WB, which detected target protein expression. ( C-D ) SERPINH1 was knocked down in U251 and LN229 cells using siRNA, and the expression level of the target protein was subsequently detected by immunofluorescence. ( E ) Immunohistochemistry detected the expression level of the target protein in human glioma tissue microarrays. ( F-H ) Correlation analysis shows a significant positive correlation between the expression levels of SERPINH1 and those of STAT3, p-STAT3, and MCL1 in human gliomas.

Article Snippet: Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence were performed using primary antibodies against SERPINH1 (proteintech, 10875-1-AP, China), MYD88 (proteintech, 23230-1-AP, China), LY96 (proteintech, 11784-1-AP, China), PDIA3 (proteintech, 15967-1-AP, China), GAPDH (proteintech, 80570-1-RR, China).

Techniques: Knockdown, Expressing, Immunofluorescence, Immunohistochemistry